You do realize don't you, that you are not in fact Reviewer #3, right? There are only TWO reviewers on this manuscript, thereby absolving you of any obligation to fulfill that detestable role. So, wherefore the vitriol? I mean seriously, I addressed all your points from the first round, and now in this second round you're saying that I failed to demonstrate something that you praised the everliving fuck out of on the first submission. I'm confused. Are you off your meds?
Also, this one thing that you have been stuck on since the first revision - I've done everything you said I should do to placate your concerns on that matter in the resubmission. You still don't like it. I'm beginning to think that you're just being cranky and obtuse. We've talked about this - if you want me to show that something is NOT expressed, you're going to have to be happy with a negative result and a fuckton of controls to show that this result is valid. There is NO WAY I can show you a positive detection of a gene product that isn't there. You do understand how such a request defies the rules of logic, right?
I'm getting a little tired of all this. Please stop fucking with me.