Dear Favorite Antibody,
Do you know why you are my favorite antibody? Because you work. Every time. I can put on you any tissue, in all permutations of blocking solution, and you always, without fail, give me clean clear bright signal, and no background to speak of. I brag about you in the lab whenever I do immunos. Did you know that? I praise your consistency to my colleagues, and I tell them all the time how much better you are than any other antibody that I have ever had.
Until today. Today you didn't work. No signal. None. What gives?
Whatever it is, I hope you get it sorted out soon. I really miss those images we used to do together.